The effects of Reynolds number across 𝑅𝑒 =1000, 2500, 5000 and 10 000 on separated flow over a two-dimensional NACA0012 airfoil at an angle of attack of 𝛼 =14∘ are investigated through biglobal resolvent analysis. We identify modal structures and energy amplifications over a range of frequencies, spanwise wavenumbers, and values of the discount parameter, providing insights across various time scales. Using temporal discounting, we find that the shear-layer dynamics dominates over short time horizons, while the wake dynamics becomes the primary amplification mechanism over long time horizons. Spanwise effects also appear over long time horizons, sustained by low frequencies. The low-frequency and high-wavenumber structures are found to be dominated by elliptic mechanisms within the recirculation region. At a fixed angle of attack and across the Reynolds numbers, the response modes shift from wake-dominated structures at low frequencies to shear-layer-dominated structures at higher frequencies. The frequency at which the dominant mechanism changes is independent of the Reynolds number. Comparisons at a different angle of attack (𝛼 =9∘) show that the transition from wake to shear-layer dynamics with increasing frequency only occurs if the unsteady flow is three-dimensional. We also study the dominant frequencies associated with wake and shear-layer dynamics across the angles of attack and Reynolds numbers, and confirm characteristic scaling laws from the literature.